rabbit anti ampar Search Results


anti-ampar rabbit monoclonal antibody  (Brickell Biotech)
90
Brickell Biotech anti-ampar rabbit monoclonal antibody
ATP11B increases glutamate release and the expression of glutamate receptors in hippocampal neurons. ( A ) The biochemical detection of glutamate released by primary hippocampal neurons into the culture medium. ( B ) The mRNA levels of the glutamate receptors, Gria1 , Gria2 , Gria3 , Gria4, and Grin1 , were assessed by qPCR in primary hippocampal neurons from the following four groups: Control, ATP11B overexpression (ATP11B), shRNA-Control, and ATP11B silencing (shRNA-ATP11B). Gapdh was used for the internal control. ( C ) The qPCR products were separated on the agarose gel. ( D ) Western blotting detection of the protein expression levels of <t>AMPAR</t> and GRIN1 following up- or downregulation of Atp11b in primary hippocampal neurons. ( E ) Data are expressed as a percentage of the control relative to GAPDH after background subtraction. Data are expressed as mean ± SEM. One-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001.
Anti Ampar Rabbit Monoclonal Antibody, supplied by Brickell Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
anti-ampar rabbit monoclonal antibody - by Bioz Stars, 2026-02
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ATP11B increases glutamate release and the expression of glutamate receptors in hippocampal neurons. ( A ) The biochemical detection of glutamate released by primary hippocampal neurons into the culture medium. ( B ) The mRNA levels of the glutamate receptors, Gria1 , Gria2 , Gria3 , Gria4, and Grin1 , were assessed by qPCR in primary hippocampal neurons from the following four groups: Control, ATP11B overexpression (ATP11B), shRNA-Control, and ATP11B silencing (shRNA-ATP11B). Gapdh was used for the internal control. ( C ) The qPCR products were separated on the agarose gel. ( D ) Western blotting detection of the protein expression levels of AMPAR and GRIN1 following up- or downregulation of Atp11b in primary hippocampal neurons. ( E ) Data are expressed as a percentage of the control relative to GAPDH after background subtraction. Data are expressed as mean ± SEM. One-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Journal of Molecular Cell Biology

Article Title: ATP11B deficiency leads to impairment of hippocampal synaptic plasticity

doi: 10.1093/jmcb/mjz042

Figure Lengend Snippet: ATP11B increases glutamate release and the expression of glutamate receptors in hippocampal neurons. ( A ) The biochemical detection of glutamate released by primary hippocampal neurons into the culture medium. ( B ) The mRNA levels of the glutamate receptors, Gria1 , Gria2 , Gria3 , Gria4, and Grin1 , were assessed by qPCR in primary hippocampal neurons from the following four groups: Control, ATP11B overexpression (ATP11B), shRNA-Control, and ATP11B silencing (shRNA-ATP11B). Gapdh was used for the internal control. ( C ) The qPCR products were separated on the agarose gel. ( D ) Western blotting detection of the protein expression levels of AMPAR and GRIN1 following up- or downregulation of Atp11b in primary hippocampal neurons. ( E ) Data are expressed as a percentage of the control relative to GAPDH after background subtraction. Data are expressed as mean ± SEM. One-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Neuralbasal (Gibco), B-27 (Gibco), GlutaMax (Gibco), fetal bovine serum (FBS; Invitrogen), Lipofectamine 2000 (Invitrogen), anti-GluR1 mouse monoclonal antibody (Santa Cruz), anti-AMPAR rabbit monoclonal antibody (BBI), anti-GRIN1 rabbit monoclonal antibody (BBI), 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen), anti-GAPDH mouse monoclonal antibody (Santa Cruz), and SB203580 (Selleck).

Techniques: Expressing, Over Expression, shRNA, Agarose Gel Electrophoresis, Western Blot